- The mature egg is held with a specialized holding pipette.
- A very delicate, sharp and hollow needle is used to immobilize and pick up a single sperm.
- This needle is then carefully inserted through the zona (shell of the egg) and in to the center (cytoplasm) of the egg.
- The sperm is injected in the cytoplasm and the needle is removed.
- The eggs are checked the next morning for evidence of Normal fertilization
The ICSI process differs from IVF only in the Laboratory. All the steps for the patient from the time of using hormonal injections to stimulate the production of multiple eggs till the embryo transfer remain the same for the patient. However in the Lab, the oocytes are subjected to ICSI instead of just leaving the sperms surrounding the egg and waiting for fertilization as happens in IVF.